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SARS-CoV-2 (COVID-19) Nucleocapsid Antibody [3868]
SKU: orb1273019
Description
Research Area
Infectious Disease & Virology
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, IF |
|---|---|
| Reactivity | Virus |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | 3868 |
| Immunogen | These newer COVID-19 nucleoprotein Mabs were developed using a recombinant, full-length nucleoprotein and then confirmed that they are reactive with the native virus itself. |
| Purification | Coronavirus Nucleocapsid Antibody is purified from ascites fluid or culture medium by protein A chromoatography or sequential differential precipitations. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Coronavirus Nucleocapsid Antibody is in a phosphate saline buffer (0.01M, pH 7.2) containing 0.1% sodium azide preservative. No stabilizing proteins have been added. |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−Coronavirus Nucleocapsid, SARS
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![SARS-CoV-2 (COVID-19) Nucleocapsid Antibody [3868]](/images/pub/media/catalog/product/NewWebsite/15/orb1273019_1.jpg)
Sandwich ELISA for SARS-CoV-2 (COVID-19) Matched Pair Nucleocapsid Antibodies. Antibodies: SARS-CoV-2 (COVID-19) Nucleocapsid Antibodies, orb1274588 and orb1273022. A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid antibody (orb1274588, 2 ug/ml) as capture antibody, the Nucleocapsid recombinant protein as the binding protein (orb1231585), and the anti-SARS-CoV-2 Nucleocapsid antibody (orb1273022, 0.5 ug/ml) as the detection antibody. Secondary: Goat anti-mouse IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 15.82 ng
![SARS-CoV-2 (COVID-19) Nucleocapsid Antibody [3868]](/images/pub/media/catalog/product/NewWebsite/15/orb1273019_2.jpg)
Sandwich ELISA for SARS-CoV-2 (COVID-19) Matched Pair Nucleocapsid Antibodies. Antibodies: SARS-CoV-2 (COVID-19) Nucleocapsid Antibodies, orb1274589 and orb1273019. A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid antibody (orb1274589, 2 ug/ml) as capture antibody, the Nucleocapsid recombinant protein as the binding protein (orb1231585), and the anti-SARS-CoV-2 Nucleocapsid antibody (orb1273019, 0.5 ug/ml) as the detection antibody. Secondary: Goat anti-mouse IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 4.27 ng
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Protocol Information
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
SARS-CoV-2 (COVID-19) Nucleocapsid Antibody [3868] (orb1273019)
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