SARS-CoV-2 (COVID-19) Nucleocapsid Antibody [3866]

SKU: orb1273021

Description

Images & Validation

−

Item 1 of 4

Tested Applications	ELISA, IF
Reactivity	Virus
Application Notes	Suggested starting ranges are 1:10-1:50 for IFA and 1:20-1:200 for ELISA. BEST ELISA pairs (capture-conjugate): clones 3865-3866 / 3869-3866

Key Properties

−

Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG1
Clone No.	3866
Immunogen	These newer COVID-19 nucleoprotein Mabs were developed using a recombinant, full-length nucleoprotein and then confirmed that they are reactive with the native virus itself.
Purification	Coronavirus Nucleocapsid Antibody is purified from ascites fluid or culture medium by protein A chromoatography or sequential differential precipitations.
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Coronavirus Nucleocapsid Antibody is in a phosphate saline buffer (0.01M, pH 7.2) containing 0.1% sodium azide preservative. No stabilizing proteins have been added.
Concentration	batch dependent
Disclaimer	For research use only

Alternative Names

−

Coronavirus Nucleocapsid, SARS

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

SARS-CoV-2 (COVID-19) Nucleocapsid Antibody [3866]

Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) Nucleocapsid in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) Nucleocapsid antibody (orb1273021, 1 ug/ml). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 °C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 Nucleocapsid protein was observed in the macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.

Sandwich ELISA for SARS-CoV-2 (COVID-19) Matched Pair Nucleocapsid Antibodies. Antibodies: SARS-CoV-2 (COVID-19) Nucleocapsid Antibodies, orb1273021 and orb1274175. A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid antibody (orb1273021, 2 ug/ml) as capture antibody, the Nucleocapsid recombinant protein as the binding protein (orb1231585), and the anti-SARS-CoV-2 Nucleocapsid antibody (orb1274175, 0.1 ug/ml) as the detection antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 6.9 ng

Sandwich ELISA for SARS-CoV-2 (COVID-19) Matched Pair Nucleocapsid Antibodies. Antibodies: SARS-CoV-2 (COVID-19) Nucleocapsid Antibodies, orb1273021 and orb1239996. A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid antibody (orb1273021, 5 ug/ml) as capture antibody, the Nucleocapsid recombinant protein as the binding protein (orb1231585), and the anti-SARS-CoV-2 Nucleocapsid antibody (orb1239996, 1 ug/ml) as the detection antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 6.9 ng

Sandwich ELISA for SARS-CoV-2 (COVID-19) Matched Pair Nucleocapsid Antibodies. Antibodies: SARS-CoV-2 (COVID-19) Nucleocapsid Antibodies, orb1274176 and orb1273021. A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid antibody (orb1274176, 5 ug/ml) as capture antibody, the Nucleocapsid recombinant protein as the binding protein (orb1231585), and the anti-SARS-CoV-2 Nucleocapsid antibody (orb1273021, 1 ug/ml) as the detection antibody. Secondary: Goat anti-mouse IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 2.36 ng

Quick Database Links

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Immunofluorescence

View Protocol

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

Find More Protocols