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SARS-CoV-2 (COVID-19) Nucleocapsid Antibody [3861]
SKU: orb1273032
Description
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, IF, WB |
|---|---|
| Reactivity | Virus |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | 3861 |
| Purification | SARS-CoV-2 (COVID-19, 2019-nCoV) Nucleoprotein antibody is purified from ascites fluid or culture medium by protein A chromoatography or sequential differential precipitations. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | SARS-CoV-2 (COVID-19, 2019-nCoV) Nucleoprotein antibody is in a phosphate saline buffer (0.01M, pH 7.2) containing 0.1% sodium azide preservative. No stabilizing proteins have been added. |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−Anti-SARS-CoV-2 (COVID-19, 2019-nCoV) Nucleoprotein
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![SARS-CoV-2 (COVID-19) Nucleocapsid Antibody [3861]](/images/pub/media/catalog/product/NewWebsite/15/orb1273032_1.jpg)
Western Blot Validation with Human Recombinant Protein. Loading: 50 ng per lane of human nucleocapsid recombinant protein. Antibodies: SARS-CoV-2 nucleocapsid, orb1273032, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Lane 1: 1 ug/mL Lane 2: 2 ug/mL.
![SARS-CoV-2 (COVID-19) Nucleocapsid Antibody [3861]](/images/pub/media/catalog/product/NewWebsite/15/orb1273032_2.jpg)
ELISA Test. Antibodies: SARS-CoV-2 Nucleocapsid antibody, orb1273032. An ELISA was performed using human SARS-CoV-2 Nucleocapsid recombinant protein as coating antigen and the SARS-CoV-2 Nucleocapsid antibody, as the capture antibody. Secondary: Goat anti-mouse IgG HRP conjugate at 1:20000 dilution. Detection range is from 16 ng/mL to 1000 ng/mL.
![SARS-CoV-2 (COVID-19) Nucleocapsid Antibody [3861]](/images/pub/media/catalog/product/NewWebsite/15/orb1273032_3.jpg)
ELISA Test. Antibodies: SARS-CoV-2 Nucleocapsid antibody, orb1273032. An ELISA was performed using human SARS-CoV Nucleocapsid recombinant protein as coating antigen and the SARS-CoV-2 Nucleocapsid antibody, as the capture antibody. Secondary: Goat anti-mouse IgG HRP conjugate at 1:20000 dilution. Detection range is from 8 ng/mL to 1000 ng/mL.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
SARS-CoV-2 (COVID-19) Nucleocapsid Antibody [3861] (orb1273032)
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