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SARS-CoV-2 (COVID-19) S1 RBD Detection Set
SKU: orb1227424
Description
Images & Validation
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| Tested Applications | ELISA, ICC |
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Key Properties
−| Reactivity | Virus |
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| Assay Type | Detection |
| Concentration | Antibody 1 mg/mL |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Antibodies should be stored in working aliquots at -20˚°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
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| Disclaimer | For research use only |
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ELISA Validation with SARS-CoV-2 (COVID-19) Spike RBD Protein. Antibodies: SARS-CoV-2 (COVID-19) Spike S1 RBD Antibodies. A direct ELISA was performed using SARS-CoV-2 Spike RBD recombinant protein (orb1231588) as coating antigen at 1 μg/mL and each of the 6 anti-SARS-CoV-2 (COVID-19) Spike S1 RBD antibodies as the capture antibody, following by anti-cMyc-tag antibody (orb1239964) at 1 μg/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL.
ELISA Validation with SARS-CoV-2 (COVID-19) Spike S1 Protein. Antibodies: SARS-CoV-2 (COVID-19) Spike S1 RBD Antibodies. A direct ELISA was performed using SARS-CoV-2 Spike S1 recombinant protein (orb1227612) as coating antigen at 1 μg/mL and each of the 6 anti-SARS-CoV-2 (COVID-19) Spike S1 RBD antibodies as the capture antibody, following by anti-cMyc-tag antibody (orb1239964) at 1 μg/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL. SARS-CoV-2 (COVID-19) Spike S1 RBD Antibodies can detect spike S1 protein at 3 ng/mL.
ELISA Validation with SARS-CoV-2 (COVID-19) Spike ECD Protein. Antibodies: SARS-CoV-2 (COVID-19) Spike S1 RBD Antibodies. A direct ELISA was performed using SARS-CoV-2 Spike ECD recombinant protein (orb1231547) as coating antigen at 1 μg/mL and each of the 6 anti-SARS-CoV-2 (COVID-19) Spike S1 RBD antibodies as the capture antibody, following by anti-cMyc-tag antibody (orb1239964) at 1 μg/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL.
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Protocol Information
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
SARS-CoV-2 (COVID-19) S1 RBD Detection Set (orb1227424)
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