SARS-CoV-2 (COVID-19) S1+S2 ECD (S-ECD) Recombinant Protein

SKU: orb1231603

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Description

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Tested Applications	ELISA, WB
Application Notes	This recombinant protein can be used for WB. For research use only.

Key Properties

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Source	HEK293 cells
Biological Activity	Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-COV-2 Spike S1+S2 ECD at 2μg/mL (100 μL/well) can bind Recombinant Human ACE2 with a linear range of 0.15-3.03 ng/mL
Target	S
Tag	His tag at C-terminus
Molecular Weight	132.29a
Protein Sequence	Cys15-Gln1208 with mutation in the cleavage site (683 G)
Purity	> 97% as determined by SDS-PAGE.

Storage & Handling

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Storage	This product is stable at ≤ ‑70˚°C for up to 6 months from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.
Form/Appearance	Lyophilized
Buffer/Preservatives	PBS, pH 7.4
Disclaimer	For research use only

Alternative Names

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2019-nCoV spike protein, 2019-nCoV S glycoprotein, COVID-19 S protein, Spike glycoprotein, SARS-CoV-2 Spike

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Immobilized SARS-CoV-2 (COVID-19) S1 + S2 ECD (S-ECD) recombinant protein at 2μg/mL (100 μL/well) can bind recombinant human ACE2 with a linear range of 1.5-35.1 ng/mL.

SARS-CoV-2 (COVID-19) S1 + S2 ECD (S-ECD) recombinant protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 170-220 kDa.

Quick Database Links

Gene Symbol

UniProt

P0DTC2

RefSeq:QHD43416.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

RefSeq	QHD43416.1

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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