SARS-CoV-2 (COVID-19) Spike 681P Antibody [8G10C8]

ELISA Validation of Spike 681P Antibodies with SARS-CoV-2 Spike S1 Protein.Coating Antigens: SARS-CoV-2 spike S1 proteins: WT and alpha variant (B.1.1.7, UK), 2 μg/mL, incubated at 4°C overnight. Detection Antibodies: SARS-CoV-2 Spike 681P antibody, orb1239046, dilution: 1-3000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5000, incubated at RT for 1 hr. SARS-CoV-2 spike 681P antibody (orb1239046) detected WT spike S1 protein (orb1231589), but not alpha variant spike S1 protein by ELISA.

WB Validation of Spike 681P Antibodies with SARS-CoV-2 Spike S1 Protein.Loading: 30 ng of SARS-CoV-2 spike S1 proteins: WT and alpha variant (B.1.1.7, UK). Detection Antibodies: SARS-CoV-2 Spike 681P antibody, orb1239046, 1 μg/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5000, incubated at RT for 1 hr. SARS-CoV-2 spike 681P antibody (orb1239046) detected WT spike S1 protein (orb1231589), but not alpha variant spike S1 protein by WB.

ELISA Validation of Spike 681P Antibodies with Mutant and WT Peptide.Coating Antigen: SARS-CoV-2 spike S1 peptides: WT (orb1231701) and alpha variant (B.1.1.7, UK) (orb1231664), 1 μg/mL, incubate at 4°C overnight. Detection Antibodies: SARS-CoV-2 681P antibody, orb1239046, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5000, incubated at RT for 1 hr. SARS-CoV-2 spike 681P antibody (orb1239046) detected WT peptide (681P, orb1231701), but not alpha variant spike S1 peptide (681H, orb1231664).