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SARS-CoV-2 (COVID-19) Spike (D614G) (Stable Trimer) Recombinant Protein
SKU: orb1231041
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Description
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Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Binds to the human SARS-CoV-2 receptor ACE2. Binds to anti-SARS-CoV-2 Spike antibodies in serum or plasma. |
| Target | S |
| Molecular Weight | ~190kDa (SDS-PAGE) |
| Protein Sequence | Full-length soluble SARS-CoV-2 Spike protein (aa 16-1213) including a foldon trimerization motif, mutated Furin recognition site (R682S, R685S) and two stabilizing mutations (K986P and V987P) is fused at the C-terminus to a His-tag. |
| Purity | ≥90% (SDS-PAGE) |
Storage & Handling
−| Storage | Short Term StorageL: +4°C, Long Term Storage: -20°C, Handling Advice: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. For maximum product recovery after thawing, centrifuge the vial before opening the cap. Use/Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Lyophilized. Contains PBS and 0.5% trehalose. |
| Concentration | 0.2mg/ml after reconstitution. |
| Disclaimer | For research use only |
Alternative Names
−2019-nCoV Spike Protein S
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Full-length trimeric SARS-CoV-2 Spike binds to its receptor ACE2 (human) (rec.) with high affinity.
SDS-PAGE: Coomassie staining under non-reducing conditions, showing one clear band at 190kDa and confirming purity of the protein.
SARS-CoV-2 Spike Protein (D614G) (Stable Trimer) (rec.) (His) migrates by Size Exclusion Chromatography (SEC) as a single peak.
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SARS-CoV-2 (COVID-19) Spike (D614G) (Stable Trimer) Recombinant Protein (orb1231041)
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