SARS-CoV-2 (COVID-19) Spike S1 Antibody (biotin)

SKU: orb1239026

Description

Images & Validation

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Item 1 of 3

Tested Applications	ELISA
Reactivity	Virus

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Anti-SARS-CoV-2 (COVID-19) Spike S1 Antibody (biotin) (orb1239026) was raised against a peptide corresponding to 16 amino acids near the amino terminus of SARS-CoV-2 (COVID-19) Spike S1 glycoprotein. The immunogen is located within the first 50 amino acids of SARS-CoV-2 (COVID-19) Spike S1 protein.
Target	S
Purification	SARS-CoV-2 (COVID-19) Spike S1 Antibody (biotin) is affinity chromatography purified via peptide column.
Conjugation	Biotin

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	SARS-CoV-2 (COVID-19) Spike S1 Antibody (biotin) is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Disclaimer	For research use only

Alternative Names

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SARS-CoV-2 (COVID-19) Spike S1 Antibody (biotin): Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Surface Glycoprotein, Spike protein

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SARS-CoV-2 (COVID-19) Spike S1 Antibody (biotin)

ELISA Validation of SARS-CoV-2 Spike Antibody Pair.A sandwich ELISA was performed using the anti-SARS-COV-2 Spike S1 antibodies orb1239981 (10 μg/mL) as the capture antibody. Biotin-labled anti-SARS-COV-2 Spike S1 antibodies orb1239026 (1 μg/mL) and streptavidin-HRP (0.1 μg/mL) were used for detection. Detection range is from 0.8 ng to 200 ng (SARS-CoV-2 Spike S1 Protein, orb1227612).

Sandwich ELISA for SARS-CoV-2 (COVID-19) Matched Pair Spike S1 Antibodies. Antibodies: SARS-CoV-2 (COVID-19) Spike Antibodies, orb1274178 and orb1239026. A sandwich ELISA was performed using SARS-CoV-2 Spike S1 antibody (orb1274178, 2ug/ml) as capture antibody, the Spike S1 recombinant protein as the binding protein (orb1227612), and the anti-SARS-CoV-2 Spike S1 antibody (orb1239026, 1ug/ml) as the detection antibody. Secondary: Streptavidin-HRP at 1:10000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 5.1 ng

Sandwich ELISA for SARS-CoV-2 (COVID-19) Matched Pair Spike S1 Antibodies. Antibodies: SARS-CoV-2 (COVID-19) Spike Antibodies, orb1274177 and orb1239026. A sandwich ELISA was performed using SARS-CoV-2 Spike S1 antibody (orb1274177, 2ug/ml) as capture antibody, the Spike S1 recombinant protein as the binding protein (orb1227612), and the anti-SARS-CoV-2 Spike S1 antibody (orb1239026, 1ug/ml) as the detection antibody. Secondary: Streptavidin-HRP at 1:10000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 1.96 ng

Quick Database Links

Gene Symbol

UniProt

P0DTC2

RefSeq:QHD43416

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

RefSeq	QHD43416

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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