SARS-CoV-2 (COVID-19) Spike S2 Antibody [4F10]

Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) Spike in COVID-19 Patient Lung Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 (COVID-19) Spike S2 antibody (orb1238686, 0.5 μg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°. A goat anti-mouse IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong spike protein signal was observed in macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.

Overexpression Validation in Spike Transfected 293 Cells.Loading: 5 μg per lane of 293 cell lysate. Antibodies: SARS-CoV-2 (COVID-19) Spike S2, orb1238686, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10, 000 dilution. Lane 1: 0.5 μg/mL and.Lane 2: 1 μg/mL

Immunofluorescence Validation of SARS-CoV-2 (COVID-19) Spike in 293T Cells.Immunofluorescent analysis of 4% paraformaldehyde-fixed 293T cells labeling SARS-CoV-2 (COVID-19) Spike S2 with orb1238686 at 20 μg/mL, followed by goat anti-mouse IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).

ELISA Test. Antibodies: SARS-CoV-2 (COVID-19) Spike S2 antibody, orb1238686 (1 μg/mL). A direct ELISA was performed using immunogen or control peptide as coating antigen and the anti-SARS-CoV-2 (COVID-19) Spike antibody as the capture antibody. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL.