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SARS-CoV-2 (COVID-19) Spike S2 Single Domain Antibody [P1A9]
SKU: orb1274190
Description
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA |
|---|---|
| Reactivity | Virus |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Camelus |
| Clonality | Recombinant |
| Isotype | sdAb |
| Clone No. | P1A9 |
| Immunogen | SARS-CoV-2 Spike S2 protein containing C-terminal His Tag. The protein was expressed in human 293 cells (HEK293). It contains amino acids Ser 686 - Pro 1213. |
| Target | S |
| Purification | SARS-CoV-2 (COVID-19) Spike S2 Antibody is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein. It also contains a myc-tag for detection. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | SARS-CoV-2 (COVID-19) Spike S2 Antibody is supplied in PBS. |
| Concentration | batch dependent |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−SARS-CoV-2 (COVID-19) Spike S2 Antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Surface Glycoprotein, Spike protein
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![SARS-CoV-2 (COVID-19) Spike S2 Single Domain Antibody [P1A9]](/images/pub/media/catalog/product/NewWebsite/15/orb1274190_1.jpg)
ELISA Validation with Spike Trimer Proteins of SARS-CoV-2 Variants and SARS-CoV-1 Antibodies: SARS-CoV-2 (COVID-19) Spike S2 Antibody, orb1274190. A direct ELISA was performed using Spike trimer protein of SARS-CoV-2 Variants (Delta and Omicron) and SARS-CoV-1 as coating antigens at 1 μg/mL and the anti-SARS-CoV-2 (COVID-19) Spike S2 antibody (orb1274190) as the capture antibody, following by anti-cMyc-tag antibody (orb1239964) at 1 μg/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 16 ng/mL to 2000 ng/mL.
![SARS-CoV-2 (COVID-19) Spike S2 Single Domain Antibody [P1A9]](/images/pub/media/catalog/product/NewWebsite/15/orb1274190_2.jpg)
Epitope Mapping of four Spike S2 Antibodies by Direct Competitive ELISA A direct competitive ELISA was developed for the epitope mapping using Spike trimer proteins of SARS-CoV-2 Delta variant at 1 μg/ml as the coating antigen and biotinylated Spike S2 single domain antibodies (sdAbs) as the competitive antibodies. The sdAb mixtures (biotinylated and unconjugated sdAbs) were added to ELISA plate and streptavidin-HRP was used to detect the captured biotinylated sdAbs. The assay suggests the antibodies bind to different epitopes of the Spike trimer.
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SARS-CoV-2 (COVID-19) Spike S2 Single Domain Antibody [P1A9] (orb1274190)
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