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SARS-CoV-2 (COVID-19) UK variant (B.1.1.7) S RBD (N501Y) Recombinant Protein
SKU: orb1231548
Description
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Tag | His-Avi |
| Protein Sequence | Arg319 - Phe541 (N501Y) |
| Purity | > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLCEndotoxin level is less than 1EU per ug by the LAL method. |
Storage & Handling
−| Storage | Store in working aliquots at -20˚°C. Avoid freeze/thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 20mM PB (pH 7.4). 5 % trehalose is added as protectant before lyophilization. |
| Disclaimer | For research use only |
Alternative Names
−S protein RBD, Spike glycoprotein Receptor-binding domain, S glycoprotein RBD, Spike protein RBD
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SARS-CoV-2 (COVID-19) UK variant (B.1.1.7) S RBD (N501Y) Recombinant Protein [orb1231553]
>90% as determined by SDS-PAGE
35kDa
Mammalian Cells
0.1 mg
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Immobilized SARS-CoV S protein RBD (N501Y), His Tag at 0.5 ug/ml (100ug/Well) on the plate. Dose response curve for Human ACE2, Fc Tag with the EC50 of 12.7 ng/ml determined by ELISA.
The purity of SARS-COV-2 S protein RBD (N501Y) is greater than 95% as determiend by SEC-HPLC.
Human ACE2 captured in protein A chip, can bind SARS-CoV S protein RBD (N501Y), His Tag with an affinity constant of 1.74nM as determined in SPR assap (Biacore T200).
SARS-COV2 S protein RBD (N501Y) on Tris-Bis PAGE under reduced condition. The purity os great than 95%.
Quick Database Links
RefSeq:QHD43416.1
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| RefSeq | QHD43416.1 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
SARS-CoV-2 (COVID-19) UK variant (B.1.1.7) S RBD (N501Y) Recombinant Protein (orb1231548)
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