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SARS CoV-2 full length spike protein in DIBMA Glycerol
Description
Research Area
Images & Validation
−| Tested Applications | ELISA |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | not determined |
| Target | S |
| Tag | C-terminal Rho1D4 |
| MW | 1286 amino acids (including Rho1D4 tag and linker); 142114 Da |
| Purity | >98% as determined by SDS-PAGE, see Fig. 1 A and B. PureCube Rho1D4 Agarose. |
| Protein Sequence | Full-length sequence (aa 1 – 1273),furin cleavage site “RRAR” mutated to “GSAG”;C-terminal Rho1D4 tag fused with spacer “GSSG” to protein sequence |
Storage & Handling
−| Storage | –80°C. Avoid freeze-thaw cycles |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 20 mM Hepes pH 7.5; 150 mM NaCl,: buffer does not contain free DIBMA Glycerol (DIBMA: Diisobutylene-maleic acid) |
| Concentration | batch dependent |
| Expiration Date | 6 months from date of receipt. |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
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SARS CoV-2 full length spike protein in DIBMA Glycerol

A: SDS of SPIKE in synthetic nanodisc. B: Western blot of Rhot1d4 tagged SPIKE, originated from the SDS Page seen in "A". C: Native protein Gel of purified and stabilized SPIKE in synthetic nanodisc.
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Protocol Information
SARS CoV-2 full length spike protein in DIBMA Glycerol (orb1231039)
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