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Recommended Pair - SARS-CoV-2 Nucleoprotein (NP) (85C10) rabbit mAb Biotin Conjugate Antibody
Description
Images & Validation
−| Tested Applications | ELISA |
|---|---|
| Dilution range | Used for specific, high-sensitivity detection of SARS-CoV-2 nucleocapsid protein (NP) in immunoassay. Can be paired with other NP-specific clones for detection in sandwich ELISA format. |
| Reactivity | Other |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Monoclonal |
| Isotype | Rabbit IgG1k |
| Clone No. | 85C10 |
| Immunogen | SARS-CoV-2 nucleoprotein (NP) specific peptide |
| Purification | Protein A |
| Conjugation | Biotin |
Storage & Handling
−| Storage | 2-8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 1X PBS, 0.02% NaN3 |
| Concentration | 0.5 mg/mL |
| Disclaimer | For research use only |
Alternative Names
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Microtiter wells were coated with SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) at 1 ug/mL. Biotin-conjugated rabbit monoclonal antibody 85C10 (Cat# orb1945982) was serially diluted 1:2 starting at 1 ug/mL, and shows high-sensitivity binding to COVID-19 NP antigen.

A sandwich ELISA was performed using SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) specific rabbit monoclonal antibodies: 75G5a (Cat# orb1945992) as a capture antibody and 85C10-biotin (Cat# orb1945982) as a detection antibody. COVID-19 NP was serially diluted 1:2 starting at 20 ng/mL. Rabbit monoclonal antibodies 75G5a and 85C10-biotin detected COVID-19 NP antigen at very high sensitivity as low as 19.5 pg/mL (1.9 pg).
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Recommended Pair - SARS-CoV-2 Nucleoprotein (NP) (85C10) rabbit mAb Biotin Conjugate Antibody (orb1945982)
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