SARS-CoV-2 RBD ELISA Kit

SKU: orb2975618

Description

PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for SARS-CoV-2 RBD has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any SARS-CoV-2 RBD present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-labeled SARS-CoV-2 RBD antibody is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells.Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SARS-CoV-2 RBD bound in the initial step. The color development is stopped and the intensity of the color is measured.

Images & Validation

−

Key Properties

−

Sample Types	Serum, Plasma and Cell culture supernatant.
Assay Type	Quantitative
Range	12.5 - 800 pg/mL
Sensitivity	4.92 pg/mL

Storage & Handling

−

Storage	When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20°C, the rest reagents should be store at 4°C.
Disclaimer	For research use only

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Quick Database Links

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Find More Protocols