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SARS-CoV-2 Spike P681H Antibody [7A4D12](Alpha, Mu Variant)
SKU: orb1238730
Description
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, WB |
|---|---|
| Reactivity | Virus |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a, Kappa |
| Clone No. | 7A4D12 |
| Immunogen | Anti-SARS-CoV-2 (COVID-19) P681H Mutant Specific Spike P681H antibody (orb1238730) was raised against a peptide corresponding to 12 amino acids near the center of SARS-CoV-2 Alpha variant (B.1.1.7) Spike protein. The immunogen is located within 650-700 amino acids of SARS-CoV-2 Alpha Variant (B.1.1.7, UK) Spike protein. |
| Target | S |
| Purification | SARS-CoV-2 (COVID-19) Alpha Variant (B.1.1.7, UK) Spike P681H Antibody is Protein A purified. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | SARS-CoV-2 (COVID-19) P681H Mutant Specific Spike antibody is supplied in PBS containing 0.02% sodium azide. |
| Concentration | 1 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−SARS-CoV-2 (COVID-19) P681H Mutant Specific Spike antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Surface Glycoprotein, Spike protein
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ELISA Validation of Alpha Variant Spike Antibodies with Spike S1 Protein of SARS-CoV-2 Variants.Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and omicron variant (B.1.1.529), 1 μg/mL, incubate at 4°C overnight. Detection Antibodies: SARS-CoV-2 Alpha Variant Spike antibody, orb1238730, dilution: 200-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5000, incubate at RT for 1 hr. SARS-CoV-2 alpha variant spike antibody (orb1238730) can specifically detect alpha variant spike S1 protein, but not spike S1 protein of WT and other tested variants by ELISA.
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ELISA Validation of P681H Mutant Specific Spike Antibodies with SARS-CoV-2 Alpha Variant Spike S1 Protein.Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and alpha variant (B.1.1.7, UK), 1 μg/mL, incubate at 4°C overnight. Detection Antibodies: SARS-CoV-2 Alpha Variant Spike antibody, orb1238730, dilution: 0.3-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5000, incubate at RT for 1 hr. SARS-CoV-2 P681H Mutant Specific Spike antibody (orb1238730) can specifically detect alpha variant spike S1 protein, but not WT spike S1 protein (orb1231589) by ELISA.
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WB Validation of P681H Mutant Specific Spike Antibodies with SARS-CoV-2 Alpha Variant Spike S1 Protein.Loading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and alpha variant (B.1.1.7, UK). Detection Antibodies: SARS-CoV-2 P681H Mutant Specific Spike antibody, orb1238730, 1 μg/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5000, incubate at RT for 1 hr. SARS-CoV-2 P681H Mutant Specific Spike antibody (orb1238730) can specifically detect alpha variant spike S1 protein, but not WT spike S1 protein (orb1231589) by WB.
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ELISA Validation of P681H Mutant Specific Spike Antibodies with Mutant and WT Peptide.Coating Antigen: SARS-CoV-2 spike S1 peptides, including WT (orb1231701) and alpha variant (B.1.1.7, UK) (orb1231664), 1 μg/mL, incubate at 4°C overnight. Detection Antibodies: SARS-CoV-2 P681H Mutant Specific Spike antibody, orb1238730, dilution: 0.1-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5000, incubate at RT for 1 hr. SARS-CoV-2 P681H Mutant Specific Spike antibody (orb1238730) can specifically detect alpha variant spike S1 peptide (681H, orb1231664), but not WT peptide (681P, orb1231701).
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
SARS-CoV-2 Spike P681H Antibody [7A4D12](Alpha, Mu Variant) (orb1238730)
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