SARS-CoV-2 Spike Trimer Protein, His Tag (KP.3) (MALS verified)

SKU: orb3158198

Description

SARS-CoV-2 Spike Trimer Protein, His Tag (KP.3) is expressed from human 293 cells (HEK293). It contains AA Val 16 -Pro 1213 (Accession # QHD43416.1 (T19I, R21T, LPP24-26Del, A27S, S50L, H69del, V70del, V127F, G142D, Y144del, F157S, R158G, N211del, L212I, V213G, L216F, H245N, A264D, I332V, G339H, K356T, S371F, S373P, S375F, T376A, R403K, D405N, R408S, K417N, N440K, V445H, G446S, N450D, L452W, L455S, F456L, N460K, S477N, T478K, N481K, V483del, E484K, F486P, Q493E, Q498R, N501Y, Y505H, E554K, A570V, D614G, P621S, H655Y, N679K, P681R, N764K, D796Y, S939F, Q954H, N969K, V1104L, P1143L, R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P)). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.

Images & Validation

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Application Notes	This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 137.7 kDa. The protein migrates as 150-180 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

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Expression System	HEK293
Target	Spike protein
Reactivity	Virus
Tag	C-10×His
Molecular Weight	137.7 kDa
Expression Region	Val 16 - Pro 1213
Purity	90%
Endotoxins	1.0 EU per μg
Conjugation	Unconjugated

Storage & Handling

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Storage	-20°C
Form/Appearance	Powder
Buffer/Preservatives	0.1 M Sodium citrate, pH 5.5
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

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Quick Database Links

Gene Symbol

Spike protein

RefSeq:A264, G446, R408, N969, R403, S477, R683, A570, S939, I332, K986, N440, T19, Y505, P1143, S373, N501, D405, V1104, A892, G339, S371, H245, F817, F486, T376, R158, V127, L212, S375, L455, V987, E554, H69, A899, N460, K417, V483, R685, D614, QHD43416.1, F456, V70, K356, N679, LPP24, A27, L216, P621, P681, Y144, G142, E484, D796, N481, V445, H655, N764, R21, Q493, T478, F157, Q498, Q954, N211, N450, L452, S50, A942, V213, 26

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

RefSeq

A264, G446, R408, N969, R403, S477, R683, A570, S939, I332, K986, N440, T19, Y505, P1143, S373, N501, D405, V1104, A892, G339, S371, H245, F817, F486, T376, R158, V127, L212, S375, L455, V987, E554, H69, A899, N460, K417, V483, R685, D614, QHD43416.1, F456, V70, K356, N679, LPP24, A27, L216, P621, P681, Y144, G142, E484, D796, N481, V445, H655, N764, R21, Q493, T478, F157, Q498, Q954, N211, N450, L452, S50, A942, V213, 26

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