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SHANK1 Antibody
SKU: orb67417
Featured
Description
Research Area
Disease Biomarkers, Neuroscience, Protein Biochemistry, Signal Transduction
Images & Validation
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| Tested Applications | AM, IHC, WB |
|---|---|
| Dilution Range | WB (1:1000), IHC-P (1:1000), ICC/IF (1:100) |
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | N22/21 (Formerly sold as S22-21) |
| Immunogen | Fusion protein amino acids 469-691 (SH3/PDZ domains) of rat Shank1 |
| Target | SHANK1 |
| Molecular Weight | 190-220 kDa |
| Purification | Protein G Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS pH 7.4, 50% glycerol, 0.09% sodium azide Storage buffer changes when conjugated |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−GKAP/SAPAP interacting protein, OTTHUMP00000174437, SH3 and multiple ankyrin repeat domains 1, SH3 and multiple ankyrin repeat domains protein 1, SH3/ankyrin domain gene 1, SHAN1_HUMAN, SHANK 1, Shank1, Shank1a, Somatostatin receptor interacting protein, Somatostatin receptor-interacting protein, SPANK 1, SPANK1, SSTR interacting protein, SSTR-interacting protein, SSTRIP, Synamon
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Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK1 Monoclonal Antibody, Clone S22-21. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SHANK1 Monoclonal Antibody at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SHANK1 Antibody (D) Composite.
Immunohistochemistry analysis using Mouse Anti-SHANK1 Monoclonal Antibody, Clone S22-21. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-SHANK1 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Filaggrin-like staining (upper layer aggregations of staining).
Western Blot analysis of Rat brain membrane lysate showing detection of SHANK1 protein using Mouse Anti-SHANK1 Monoclonal Antibody, Clone S22-21. Load: 15 μg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK1 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Gene Details
− No NCBI Gene data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_113939.2 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
SHANK1 Antibody (orb67417)
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