Sheep IgG F(ab')2 Antibody Fluorescein Conjugated

SKU: orb347920

Description

Sheep IgG F(ab')2 antibody (FITC)

Images & Validation

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Tested Applications	DOT, FC, FLISA, IF
Dilution range	FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
Reactivity	Sheep
Application Notes	Anti-Sheep IgG F(ab')2 Fluorescein Antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Key Properties

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Antibody Type	Secondary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Sheep IgG F(ab')2 fragment
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Sheep IgG, Sheep IgG F(ab’)2 and Sheep Serum. No reaction was observed against Sheep IgG F(c).
Conjugation	FITC

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.01% (w/v) Sodium Azide
Concentration	1.5 mg/mL
Disclaimer	For research use only

Alternative Names

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Rabbit Anti-Sheep IgG F(ab')2 fluorescein Conjugated Antibody, Rabbit Anti-Sheep IgG Fab2 fluorescein Conjugated Antibody, Rabbit Anti-Sheep IgG Fab2 Fragment Antibody FITC Conjugation

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Dot Blot results of Rabbit Anti-Sheep IgG F(ab')2 Antibody Fluorescein Conjugate. Dots are Sheep F(ab')2: (1) 100 ng, (2) 33.3 ng, (3) 11.1 ng, (4) 3.70 ng, (5) 1.23 ng. Primary Antibody: none. Secondary Antibody: Rabbit Anti-Sheep IgG F(ab')2 Antibody FITC at 1 ug/ml in orb348637 1hr RT.

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