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Sheep IgG Fc Antibody Pre-Adsorbed
SKU: orb347916
Description
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| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution range | ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000 |
| Reactivity | Sheep |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Sheep IgG F(c) fragment |
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Sheep IgG, Sheep IgG F(c) and Sheep Serum. No reaction was observed against Sheep IgG F(ab). This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human serum proteins, but antibodies may cross-react with immunoglobulins from other species. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Concentration | 1.0 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−Rabbit Anti-Sheep IgG F(c) Antibody, Rabbit Anti-Sheep IgG Fc Antibody, Rabbit Anti-Sheep IgG Fc Fragment Antibody
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ELISA Results of Rabbit Anti-Sheep IgG F(c) Antibody (Min X Human Serum Proteins) tested against purified Sheep IgG F(c) Min Hu. Each well was coated in duplicate with 1.0 µg of Sheep IgG F(c) (p/n orb346368) [Red Line] and Human IgG (p/n orb346219) [Green Line]. The working dilution of Sheep IgG F(c) is 1:16300. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer, Goat Anti-Rabbit IgG HRP conjugated (p/n orb347654) and TMB substrate (p/n orb348651).
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WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Sheep IgG Fc Antibody Pre-Adsorbed (orb347916)
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