Sheep IgG (H&L) Antibody Peroxidase Conjugated

SKU: orb347959

Description

Sheep IgG (H&L) antibody (Peroxidase)

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, IHC, WB
Dilution range	ELISA: 1:50,000 - 1:150,000, IHC: 1:1,000 - 1:5,000, WB: 1:3,000 - 1:15,000
Reactivity	Sheep
Application Notes	Anti-Sheep IgG Peroxidase conjugated antibody has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Key Properties

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Antibody Type	Secondary Antibody
Host	Donkey
Clonality	Polyclonal
Isotype	IgG
Immunogen	Anti-Sheep IgG (H&L) was produced by repeated immunization with Sheep IgG whole molecule in donkey.
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Sheep IgG and Sheep Serum.
Conjugation	HRP

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Concentration	2.0 mg/mL
Disclaimer	For research use only

Alternative Names

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Donkey Anti-Sheep IgG peroxidase Conjugated Antibody, Donkey Anti-Sheep IgG Antibody HRP Conjugation

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Sheep IgG (H&L) Antibody Peroxidase Conjugated

ELISA results of purified Donkey anti-Sheep IgG Antibody Peroxidase Conjugated tested against purified Sheep IgG. Each well was coated in duplicate with 1.0 µg of Sheep IgG (p/n orb2652774). The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate p/n orb348651.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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