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Description
Research Area
Signal Transduction
Images & Validation
−Item 1 of 3
| Tested Applications | FC, IHC-P, WB |
|---|---|
| Dilution Range | WB - 1:1000, IHC-P - 1:50-100, FC - 1:10-50 |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone No. | BTD43615 |
| Immunogen | This SHP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 526-551 amino acids from human SHP2. Antigen Region: 526-551 aa. |
| Target | PTPN11 |
| Molecular Weight | 68011 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Tyrosine-protein phosphatase non-receptor type 11, Protein-tyrosine phosphatase 1D, PTP-1D, Protein-tyrosine phosphatase 2C, PTP-2C, SH-PTP2, SHP-2, Shp2, SH-PTP3, PTPN11, PTP2C, SHPTP2
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Phospho-SHP2(Y546) Antibody [orb1788334]
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Western blot analysis of SHP2 Antibody (Y546) in 293, K562 cell line lysates (35 ug/lane). SHP2 (arrow) was detected using the purified Pab.
Flow cytometric analysis of 293 cells using SHP2 Antibody (Y546) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with SHP2 Antibody (Y546), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_002825.3, NP_542168.1 |
|---|
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
SHP2 Antibody (Y546) (orb1928476)
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