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SIGLEC15 Antibody
SKU: orb1238667
Featured
Description
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Anti-SIGLEC15 antibody (orb1238667) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human SIGLEC15. The immunogen is located within amino acids 270-320 of SIGLEC15. |
| Target | SIGLEC15 |
| Molecular Weight | Predicted: 36kDObserved: 34-36kD |
| Purification | SIGLEC15 Antibody is affinity chromatography purified via peptide column. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | SIGLEC15 Antibody is supplied in PBS containing 0.02% sodium azide. |
| Concentration | 1 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−SIGLEC15 Antibody: CD33L3, HsT1361, SIGLEC-15, CD33L3, Sialic acid-binding Ig-like lectin 15, CD33 antigen-like 3, Siglec-15
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Western Blot Validation in Human and Mouse Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: SIGLEC15, orb1238667 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Human Tissues. Loading: 15 µg of lysates per lane. Antibodies: SIGLEC15, orb1238667 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Mouse Tissues. Loading: 15 µg of lysates per lane. Antibodies: SIGLEC15, orb1238667 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Rat Tissues. Loading: 15 µg of lysates per lane. Antibodies: SIGLEC15, orb1238667 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
SIGLEC15 Antibody (orb1238667)
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