SIGLEC15 Antibody

SKU: orb1539217

Description

SIGLEC15 Antibody

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, IHC, IHC-P, WB
Dilution range	ELISA, IHC, IHC-P (5 µg/ml), WB (1 - 2 µg/ml)
Reactivity	Human, Mouse, Rat
Application Notes	Further information: SIGLEC15 antibody can be used for detection of SIGLEC15 by Western blot at 1 - 2 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL. Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Western Blot: Predicted: 36 kDa Observed: 42 kDa

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	A 15 AA synthetic peptide near the N-Terminus of human SIGLEC15. The immunogen is located within aa 50-100 of SIGLEC15.
Target	SIGLEC15
Purification	Peptide Column Affinity Chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS containing 0.02% Sodium Azide. PBS containing 0.02% Sodium Azide
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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SIGLEC15, CD33 antigen-like 3, CD33L3, HsT1361, SIGLEC-15, CD33 molecule-like 3

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Loading: 15 µg of lysates per lane. Antibodies: SIGLEC15, (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Quick Database Links

Gene Symbol

SIGLEC15

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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IHC-P

Immunohistochemistry Paraffin

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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