SIK1 rabbit pAb Antibody

SKU: orb767770

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Description

Rabbit polyclonal antibody to SIK1

Images & Validation

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Item 1 of 3

Tested Applications	IHC, WB
Dilution range	WB 1:500-2000;IHC-p 1:50-300
Reactivity	Human, Rat

Key Properties

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Clonality	Polyclonal
Isotype	IgG
Immunogen	The antiserum was produced against synthesized peptide derived from human SIK. AA range:148-197
Molecular Weight	85
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Anti-SIK1 antibody, anti-SIK antibody, anti-SNF1LK antibody, anti-Serine/threonine-protein kinase SIK1 antibody, anti-Salt-inducible kinase 1 antibody, anti-SIK-1 antibody, anti-Serine/threonine-protein kinase SNF1-like kinase 1 antibody, anti-Serine/threonine-protein kinase SNF1LK antibody

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Western Blot analysis of COLO cells using SIK1 Polyclonal Antibody.

Western blot analysis of lysates from COLO205 cells, treated with PMA 125ng/ml 30', using SIK Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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