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IL2RA Antibody

SKU: orb1272673

Description

IL2RA Antibody

Images & Validation

Tested ApplicationsELISA, NeA, WB
ReactivityHuman
Application Notes
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hsIL-2Rα (10.0 μg/mL), a concentration of 1.0-1.5 μg/mL of this antibody is required, in the presence of 0.5 ng/mL recombinant hIL-2. ELISA:In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 100 pg/mL of recombinant human sIL-2Rα when used in conjunction with compatible secondary reagents. Western Blot:To detect hsIL-2Rα by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsIL-2Rα is 2.0-4.0 ng/lane, under reducing or non-reducing conditions

Key Properties

Antibody TypePrimary Antibody
HostMouse
ClonalityMonoclonal
Clone No.SB-647
ImmunogensIL-2Ra antibody is produced in mice using highly pure (>98%) recombinant human soluble IL-2 Receptor alpha as the immunizing antigen.
TargetIL2RA
PurificationThis IgG2aK antibody was purified from cell culture by Protein G affinity chromatography.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLyophilized
Concentrationbatch dependent
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Interleukin-2 receptor subunit alpha, TAC antigen, IL-2 receptor subunit alpha, CD25, IL2R, TCGFR, IDDM10

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IL2RA Antibody

This antibody stained frozen human tonsil. The recommended concentration is 5.0 ug/ml at room temperature. A labeled polymer detection system was used with DAB chromogen. Optimal concentrations and conditions may vary.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product
RefSeqP01589

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Datasheet
Product Information
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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

IL2RA Antibody (orb1272673)

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Available Sizes

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0.5 mg
$ 730.00
DispatchUsually dispatched within 5-10 working days
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