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SIM1 Antibody (N-term)
SKU: orb1937049
Description
Images & Validation
−Item 1 of 4
| Tested Applications | IHC-P, WB |
|---|---|
| Dilution range | IHC-P-Leica - 1:250, WB - 1:2000 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Drosophila |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 85515 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Disclaimer | For research use only |
Alternative Names
−BHLHE14
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Anti-SIM1 Antibody (N-term) at 1:2000 dilution + A549 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 86 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemical analysis on paraffin-embedded Human brain tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:250) for 15min at room temperature. Leica Bond Polymer Refine Detection was used as the secondary Antibody.
Immunohistochemical analysis on paraffin-embedded Human kidney tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:250) for 15min at room temperature. Leica Bond Polymer Refine Detection was used as the secondary Antibody.
Western blot analysis of lysate from 293T cell line, using SIM1 Antibody (N-term). diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary Antibody. Lysate at 35 ug.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
SIM1 Antibody (N-term) (orb1937049)
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