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SIRP Alpha Rabbit Polyclonal Antibody
SKU: orb4840
Featured
Description
Research Area
Inflammation, Inflammatory Mediators, Neurology, Neuroscience, Signal Transduction, Synapse Markers
Images & Validation
−Item 1 of 2
| Tested Applications | FC, WB |
|---|---|
| Dilution Range | WB=1:500-2000, Flow-Cyt=1μg /test |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Bovine, Canine, Equine, Porcine |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human CD172a (151-250/504aa) |
| Target | SIRPA |
| Molecular Weight | 56 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Signal regulatory protein alpha type 1; BIT; Brain Ig like molecule with tyrosine based activation motifs; Brain immunoglobulin like molecule with tyrosine based activation motifs; CD172 antigen like family member A; CD172a; CD172a antigen; Inhibitory receptor SHPS1; Macrophage fusion receptor; MFR; MYD 1; Myd 1; MYD1; p84; Protein tyrosine phosphatase non receptor type substrate 1; PTPNS1; SHP Substrate 1; SHPS1; Signal regulatory protein alpha 2; Signal regulatory protein alpha 3; Signal regulatory protein alpha; Signal Regulatory Protein alpha Type 1; Signal regulatory protein alpha type 2; SIRP; SIRPA; SIRPalpha; SIRPalpha1; SIRPalpha2; SIRPalpha3; Tyrosine phosphatase SHP substrate 1; Tyrosine protein phosphatase non receptor type substrate 1; Tyrosine protein phosphatase non receptor type substrate; CD172a.
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Blank control (blue): U937 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-SIRP antibody (orb4840), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.
Sample: Lane 1: Mouse Brain Lysates, Lane 2: Mouse Cerebellum Lysates, Lane 3: Mouse Spleen Lysates, Lane 4: Mouse Placenta Lysates, Lane 5: Rat Brain Lysates, Lane 6: Rat Cerebellum Lysates, Primary: Anti-SIRP Alpha (orb4840) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 56kDa, Observed band size: 56kDa.
Quick Database Links
Gene Symbol
SIRPA
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
SIRP Alpha Rabbit Polyclonal Antibody (orb4840)
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