SIRT1 Mouse Monoclonal Antibody

SKU: orb1974721

Description

Research Area

Cell Biology

Images & Validation

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Item 1 of 1

Tested Applications	ICC, IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500
Reactivity	Human

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Recombinant
Isotype	IgG
Clone No.	7C5
Immunogen	KLH conjugated synthetic peptide derived from human SIRT1
Target	SIRT1
Molecular Weight	120 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Preservative: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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SIR2; SIR2L1; SIR2alpha; Sir2a; SIR1_HUMAN; SIRT1; hSIRT1; NAD-dependent protein deacylase sirtuin-1; Regulatory protein SIR2 homolog 1; SIR2-like protein 1 (hSIR2); 2.3.1.286; SIR1_MOUSE; SIR2-like protein 1; SIR2alpha (Sir2 | mSIR2a);

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Sample: Lane 1: Human HeLa cell lysates, Lane 2: Human Jurkat cell lysates, Lane 3: Human A549 cell lysates, Primary: Anti-SIRT1 (orb1974721) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 120 kDa, Observed band size: 120 kDa.

Quick Database Links

Gene Symbol

SIRT1

UniProt

Q96EB6

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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ICC

Immunocytochemistry

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