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Featured
KO/KD
Validated
Validated
Description
Images & Validation
−Item 1 of 5
| Tested Applications | IHC, KO/KD Validated, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human SIRT3 (NP_036371.1). |
| Target | SIRT3 |
| Molecular Weight | Observed: 28kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−SIRT3 Antibody: SIR2L3, SIR2L3, NAD-dependent protein deacetylase sirtuin-3, mitochondrial, Regulatory protein SIR2 homolog 3, hSIRT3
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Western blot analysis of extracts of SW620 cells, using SIRT3 antibody (orb1274444) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Western blot analysis of extracts from normal (control) and SIRT3 knockout (KO) HeLa cells, using SIRT3 antibody (orb1274444) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunohistochemistry of paraffin-embedded rat kidney using SIRT3 antibody (orb1274444) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using SIRT3 antibody (orb1274444) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using SIRT3 antibody (orb1274444) at dilution of 1:100 (40x lens).
Quick Database Links
Gene Symbol
SIRT3
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
SIRT3 Antibody (orb1274444)
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