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SKI II

SKU: orb1300568

Description

SKI-II is a potent and selective non-ATP-competitive inhibitor of sphingosine kinase 1 (SphK1) with an IC50 of 0.5 µM, showing no activity against PKCα, PI3K, or ERK2. This tool compound is widely used in vitro and in vivo to investigate the S1P signaling pathway in cancer, inflammation, and angiogenesis research.

Research Area

Cell Biology, Pharmacology & Drug Discovery, Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

Key Properties

CAS Number312636-16-1
MW302.78
Purity99.97%
FormulaC15H11ClN2OS
SMILESOc1ccc(Nc2nc(cs2)-c2ccc(Cl)cc2)cc1
TargetWnt/beta-catenin,S1P Receptor,Apoptosis
SolubilityDMSO:40 mg/mL (132.11 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (6.61 mM)

Bioactivity

Target IC50
SPHK:0.5 μM
In Vivo
Consistent with its role in reducing S1P levels, SKI II induces apoptosis in T24 cells. In JC cells, SKI II decreases S1P formation in a concentration-dependent manner (IC50: 12 μM). Additionally, in the breast cancer cell line MDA-MB-231, SKI II significantly inhibits endogenous SK activity. Among various human cancer cell lines, including T-24, MCF-7, MCF-7/VP, and NCI/ADR, SKI II demonstrates notable antiproliferative effects (IC50: 4.6/1.2/0.9/1.3 μM). Furthermore, SKI II reverses the cisplatin resistance in SGC7901/DDP by downregulating P-gp expression and inducing apoptosis through the downregulation of SPHK1.
In Vitro
SKI II (50 mg/kg, i.p.) improves bronchial smooth muscle hyperreactivity induced by antigens in mice by inhibiting the production of endogenous sphingosine-1-phosphate. In a homologous Balb/c mouse model with JC mammary carcinoma cells, SKI II (50 mg/kg, i.p./p.o.) significantly reduces tumor growth without evident toxicity or weight loss compared to the control group.
Cell Research
T24, MCF-7, MCF-7/VP, and NCI/ADR cells are plated into 96-well tissue culture plates at ~15% confluency. After 24 hours, cells are treated with various concentrations of inhibitors. After an additional 48 hours, cell survival is assayed using the sulforhodamine B assay.(Only for Reference)

Storage & Handling

StoragePowder: -20°C for 3 years | Shipping with blue ice/Shipping at ambient temperature.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

S1PReceptor, S1P Receptor, SKI II, Sphingosine kinase, SphK-I2, SphK1, SphK, Wnt/b-catenin, Wnt, Wnt/β-catenin, Wnt/betacatenin, βcatenin, inhibit, Inhibitor, beta-catenin, betacatenin, bcatenin, Apoptosis

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Key Properties

No computed properties available.

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SKI II (orb1300568)

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% DMSO +
%+
% Tween 80 +
%

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10 mg
$ 80.00
25 mg
$ 110.00
50 mg
$ 160.00
100 mg
$ 240.00
200 mg
$ 400.00
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