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SLC2A4 Antibody

SKU: orb1248908

Description

SLC2A4 Antibody

Images & Validation

Tested ApplicationsELISA, IHC, WB
ReactivityHuman, Mouse, Rat
Predicted ReactivityBovine, Porcine
Application Notes
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 45kDa band observed in Mouse and Rat Heart lysates (calculated MW of 54.8kDa according to Human NP_001033.1 and Mouse NP_033230.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Fischer et al, J Biol Chem. 1997 Mar 14;272(11):7085-92.; PMID: 9054401). Recommended concentration: 0.3-1µg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Skeletal Muscle. Recommended concentration: 3.75µg/mL.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-TELEYLGPDEND
TargetSLC2A4
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

SLC2A4, solute carrier family 2 (facilitated glucose transporter), member 4, GLUT4, glucose transporter 4, insulin-responsive glucose transporter type 4

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SLC2A4 Antibody

orb1248908 (1 ug/ml) staining of Mouse Heart lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

SLC2A4 Antibody

orb1248908 (3.75 ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

SLC2A4 Antibody (orb1248908)

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Available Sizes

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0.1 mg
$ 920.00
DispatchUsually dispatched within 5-10 working days
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