SMAD4 Antibody (T277)

SKU: orb1928925

Description

Research Area

Cancer Biology, Cardiovascular Research, Infectious Disease & Virology, Metabolism Research, Signal Transduction

Images & Validation

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Item 1 of 4

Tested Applications	IF, WB
Dilution Range	WB - 1:1000
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Molecular Weight	60439 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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DPC4, MADH4

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Western blot analysis of SMAD4 Antibody (T277) (arrow) using rabbit polyclonal SMAD4 Antibody (T277). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SMAD4 gene (Lane 2).

Anti-SMAD4 Antibody (T277) at 1:2000 dilution + Neuro-2a whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 60 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Confocal immunofluorescent analysis of SMAD4 Antibody (T277) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

All lanes: Anti-SMAD4 Antibody (T277) at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: Jurkat whole cell lysate. Lane 3: Neuro-2a whole cell lysate. Lane 4: NIH/3T3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 60 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

UniProt

Q13485

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Immunofluorescence

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