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SMAD5 Antibody
SKU: orb69361
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Description
Images & Validation
−Item 1 of 4
| Tested Applications | FC, ICC, IHC, WB |
|---|---|
| Dilution Range | WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; IF/ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000 |
| Reactivity | Human, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | 3H9B1 |
| Immunogen | Purified recombinant fragment of human SMAD5 expressed in E. Coli. |
| Target | SMAD5 |
| Molecular Weight | 52kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody in PBS with 0.05% sodium azide. |
| Concentration | Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Dwfc; JV5-1; MADH5; DKFZp781C1895; DKFZp781O1323; SMAD5
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Flow cytometric analysis of Jurkat cells using SMAD5 antibody
Immunohistochemical analysis of paraffin-embedded brain tissues (left) and lung cancer tissues (right) using SMAD5 antibody
Immunofluorescence analysis of NTERA-2 cells using SMAD5 antibody
Wes
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
ICC
Immunocytochemistry
SMAD5 Antibody (orb69361)
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