SOD2 Mouse Monoclonal Antibody

SKU: orb500056

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Description

Images & Validation

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Item 1 of 5

Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Human, Mouse
Predicted Reactivity	Mouse

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1, k
Clone No.	6C11
Immunogen	Recombinant human SOD protein
Target	SOD2
Molecular Weight	22 kDa
Purification	Affinity purified by Protein G
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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IPO B; Manganese SOD; Superoxide Dismutase 2; Manganese superoxide dismutase; Mn SOD; MNSOD; SOD 2; SOD-2; SOD2; Superoxide dismutase [Mn] mitochondrial; Superoxide dismutase [Mn] mitochondrial precursor; Superoxide dismutase 2 mitochondrial; SODM_HUMAN.

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Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SOD2) Monoclonal Antibody, Unconjugated (orb500056) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SOD2) Monoclonal Antibody, Unconjugated (orb500056) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human pancreatic cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SOD2) Monoclonal Antibody, Unconjugated (orb500056) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: Lane 1: Hela (Human) Cell Lysate at 30 ug, Lane 2: 293T (Human) Cell Lysate at 30 ug, Lane 3: Raw264.7 (Mouse) Cell Lysate at 30 ug, Primary: Anti-SOD2 (orb500056) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 22 kD, Observed band size: 22 kD.

Sample: Siha (Human) Cell Lysate at 30 ug, HepG2 (Human) Cell Lysate at 30 ug, K562 (Human) Cell Lysate at 30 ug, U251 (Mouse) Cell Lysate at 30 ug, Primary: Anti-SOD2 (orb500056) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 22 kD, Observed band size: 22 kD.

Quick Database Links

Gene Symbol

SOD2

UniProt

P04179

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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