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Sodium Potassium ATPase Mouse Monoclonal Antibody
SKU: orb704302
Featured
Description
Images & Validation
−Item 1 of 19
| Tested Applications | IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution range | WB=1:2000-10000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1, k |
| Clone No. | 2G11 |
| Immunogen | Recombinant human Sodium Potassium ATPase (551-850/1023aa) |
| Target | ATP1A1 |
| Molecular Weight | 113 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS(pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−ATP1A1; alpha 1 Sodium Potassium ATPase; A1A1; AT1A1; AT1A1_HUMAN; Atpa-1; ATPase Na+/K+transporting alpha 1 polypeptide; ATPase Na+/K+transporting subunit alpha 1; BC010319; EC 3.6.3.9; MGC3285; MGC38419; MGC51750; Na K ATPase alpha A catalytic polypeptide; Na K ATPase catalytic subunit alpha A protein; Na(+)/K(+) ATPase 1; Na(+)/K(+) ATPase alpha-1 subunit; Na+, K+ATPase alpha subunit; Na+/K+ATPase alpha 1 subunit; Na+/K+ATPase 1; Na, K ATPase alpha 1 subunit; Nkaa1b; Sodium potassium ATPase alpha 1 polypeptide; Sodium pump 1; Sodium pump subunit alpha-1; sodium-potassium ATPase catalytic subunit alpha-1; Sodium/potassium-transporting ATPase subunit alpha-1.
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Paraformaldehyde-fixed, paraffin embedded Human Fundus, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (orb704302) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
25 ug total protein per lane of various lysates probed with Sodium Potassium ATPase monoclonal antibody, unconjugated (orb704302) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (orb704302) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (orb704302) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (orb704302) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Heart, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (orb704302) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (orb704302) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (orb704302) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (orb704302) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Heart, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (orb704302) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (orb704302) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (orb704302) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Stomach, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (orb704302) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (orb704302) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (orb704302) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Heart, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (orb704302) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (orb704302) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Liver, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (orb704302) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Stomach, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (orb704302) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
Quick Database Links
Gene Symbol
ATP1A1
UniProt
UniProt Details
− No UniProt data available
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
Sodium Potassium ATPase Mouse Monoclonal Antibody (orb704302)
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