Recombinant SOX11 Antibody

SKU: orb699754

Description

Recognizes a protein of ~47kDa, identified as SOX11. This MAb is highly specific and does not cross-react with other members of the SOX-family. Mantle cell lymphoma (MCL) accounts for 5% to 10% of mature B-cell neoplasms and is an aggressive disease genetically characterized by overexpression of Cyclin D1. Cyclin D1 overexpression is the hallmark of MCL. However, approximately 5%-10% of MCLs lack Cyclin D1 expression and may be misdiagnosed. Almost all Cyclin D1-positive as well as Cyclin D1-negative MCL, show overexpression of SOX-11. The detection of this transcription factor is a useful biomarker for identifying true cyclin D1-negative MCL.

Research Area

Developmental Biology, Epigenetics

Images & Validation

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Tested Applications	IHC-P
Dilution Range	Immunohistochemistry (FFPE): 2-4ug/ml for 30 minutes at RT
Reactivity	Human
Application Notes	Optimal dilution of the recombinant SOX11 antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	SOX11/3235R
Immunogen	A portion of amino acids 300-400 from the human protein was used as the immunogen for the recombinant SOX11 antibody.
Purification	Protein A affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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IHC staining of FFPE human mantle cell lymphoma with recombinant SOX11 antibody (clone SOX11/3235R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human cervical carcinoma with recombinant SOX11 antibody (clone SOX11/3235R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Quick Database Links

UniProt

P35716

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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