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SOX2 Antibody

SKU: orb2635108

Description

SOX2 is required for stem cell maintenance in the central nervous system, and it also regulates gene expression in the stomach. SOX2 is necessary for regulating multiple transcription factors that affect Oct 3/4 expression. An essential function of SOX2 is to stabilize embryonic stem cells in a pluripotent state by maintaining the requisite level of Oct 3/4 expression. Reportedly, SOX2 is associated with aggressive phenotypes of breast, head and neck, gastric, colorectal, bladder, and small cell lung cancers. However, SOX2 is expressed in a high percentage of lung squamous cell carcinomas and has been shown to be an independent favorable prognostic marker.

Images & Validation

Tested ApplicationsIHC-P
Dilution RangeImmunohistochemistry (FFPE): 1-2ug/ml
ReactivityHuman
Application Notes
Optimal dilution of the SOX2 antibody should be determined by the researcher.

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityRecombinant
IsotypeRabbit IgG
Clone No.SOX2/4267R
ImmunogenA recombinant fragment of human SOX2 protein was used as the immunogen for the SOX2 antibody.
PurificationProtein A/G affinity
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

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SOX2 Antibody

IHC staining of FFPE human tonsil tissue with SOX2 antibody (clone SOX2/4267R) at 2 ug/ml in PBS for 30 min RT. Strong nuclear staining observed. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SOX2 Antibody

IHC staining of FFPE human cervical tissue with SOX2 antibody (clone SOX2/4267R) at 2 ug/ml in PBS for 30 min RT. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

UniProt Details

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Protocol Information

IHC-P
Immunohistochemistry Paraffin
View Protocol

SOX2 Antibody (orb2635108)

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Available Sizes

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100 μg
$ 750.00
DispatchUsually dispatched within 5-10 working days
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