Recombinant SOX2 Antibody

SKU: orb639557

Description

Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. [UniProt]

Research Area

Developmental Biology, Epigenetics

Images & Validation

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Tested Applications	IHC-P, WB
Dilution Range	ELISA (order BSA-free format for coating),Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Reactivity	Human
Application Notes	The stated application concentrations are suggested starting points. Titration of the SOX2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	SBX2-1R
Immunogen	Amino acids 176-305 were used as the immunogen for the recombinant SOX2 antibody.
Purification	Protein A affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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IHC staining of FFPE human cervical carcinoma with recombinant SOX2 antibody (clone SBX2-1R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Western blot testing of NCCIT lysate with recombinant SOX2 antibody (clone SBX2-1R). Predicted molecular weight ~34 kDa.

Quick Database Links

UniProt

P48431

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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