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SOX4 Antibody

SKU: orb1410067

Description

Mouse monoclonal antibody to SOX4 (Master Regulator of Epithelial-Mesenchymal Transition)

Research Area

Cardiovascular Research, Cell Biology, Signal Transduction

Images & Validation

Tested ApplicationsFC, IF, IHC, WB
ReactivityHuman

Key Properties

HostMouse
ClonalityMonoclonal
IsotypeIgG2b
Clone No.PCRP-SOX4-1D6
ImmunogenRecombinant fragment (around aa45-130) of human SOX4 protein
TargetSOX4
Source/Expression SystemBioreactor
Molecular Weight47kDa
PurificationProtein A
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

SOX; SRY (sex determining region Y) box 4; SRY box containing gene 4; SRY related HMG box gene 4; Transcription factor SOX-4

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SOX4 Antibody

Analysis of Protein Array containing more than 19000 full-length human proteins using SOX4 Mouse Monoclonal Antibody (PCRP-SOX4-1D6). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

SOX4 Antibody

Flow Cytometric Analysis of PFA-fixed HeLa cells. SOX4 Mouse Monoclonal Antibody (PCRP-SOX4-1D6) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

SOX4 Antibody

Formalin-fixed, paraffin-embedded human breast carcinoma stained with SOX4 Mouse Monoclonal Antibody (PCRP-SOX4-1D6). HIER: Tris/EDTA, pH9.0, 45 min. 2°: HRP-polymer, 30 min. DAB, 5 min.

SOX4 Antibody

Formalin-fixed, paraffin-embedded human colon carcinoma stained with SOX4 Mouse Monoclonal Antibody (PCRP-SOX4-1D6). HIER: Tris/EDTA, pH9.0, 45 min. 2°: HRP-polymer, 30 min. DAB, 5 min.

SOX4 Antibody

Immunofluorescence Analysis of PFA-fixed HeLa cells stained using SOX4 Mouse Monoclonal Antibody (PCRP-SOX4-1D6) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

SOX4 Antibody

SDS-PAGE Analysis Purified SOX4 Mouse Monoclonal Antibody (PCRP-SOX4-1D6). Confirmation of Purity and Integrity of Antibody.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol

SOX4 Antibody (orb1410067)

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Available Sizes

Select a size below

20 μg
$ 370.00
100 μg
$ 680.00
100 μg (without BSA and Azide)
$ 680.00
DispatchUsually dispatched within 5-10 working days
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