SPARC Rabbit Polyclonal Antibody

SKU: orb1291522

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Description

Research Area

Cancer Research, Cell Biology, Cell Cycle, Osteogenesis, Receptor Tyrosne Kinases

Images & Validation

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Item 1 of 5

Tested Applications	ELISA, IF, IHC-Fr, IHC-P, WB
Dilution range	WB=1:500-2000, IHC-P=1:400-800, IHC-F=1:400-800, IF=1:100-500, ELISA=1:5000-10000
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Human

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant human SPARC protein (18-303/303aa)
Target	SPARC
Molecular Weight	33 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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AA517111; Basement membrane protein 40; BM 40; BM40; Cysteine rich protein; hm:zeh0062; MGC128090; ON; Osteonectin; Secreted acidic cystein rich glycoprotein; Secreted protein acidic and rich in cysteine; Secreted protein acidic cysteine rich(osteonectin); Secreted protein acidic cysteine rich; SPRC; SPRC_HUMAN.

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Measured by its binding ability in a indirect ELISA. Immobilized Human SPARC protein, His Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Human SPARC Antibody, the EC50 is 21.47 ng/ml.

Measured by its binding ability in a indirect ELISA. Immobilized Human SPARC protein, His Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Human SPARC Antibody, the EC50 is 72.97 ng/ml.

Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SPARC) Polyclonal Antibody, Unconjugated (orb1291522) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SPARC) Polyclonal Antibody, Unconjugated (orb1291522) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Cerebellum tissue lysates, Lane 2: Mouse Kidney tissue lysates, Lane 3: Mouse B16 cell lysates, Lane 4: Rat Cerebrum tissue lysates, Primary: Anti-SPARC (orb1291522) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 33 kDa, Observed band size: 33 kDa.

Quick Database Links

Gene Symbol

SPARC

UniProt

P09486

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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