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Featured
KO/KD
Validated
Validated
Description
Research Area
Cell Biology
Images & Validation
−Item 1 of 5
| Tested Applications | IF, IHC, IP, KO/KD Validated, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-440 of human SQSTM1 / p62 (NP_001135770.1). |
| Target | SQSTM1 |
| Molecular Weight | Observed: 62kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−SQSTM1 Antibody: p60, p62, A170, OSIL, PDB3, ZIP3, p62B, ORCA, Sequestosome-1, EBI3-associated protein of 60 kDa, EBIAP
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Western blot analysis of extracts of various cell lines, using SQSTM1 antibody (orb1274537) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Western blot analysis of extracts from normal (control) and SQSTM1/p62 knockout (KO) 293T cells, using SQSTM1/p62 antibody (orb1274537) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human esophagus using SQSTM1/p62 antibody (orb1274537) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using SQSTM1/p62 antibody (orb1274537) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 100 ug extracts of HepG2 cells using 3 ug SQSTM1/p62 antibody (orb1274537). Western blot was performed from the immunoprecipitate using SQSTM1/p62 antibody (orb1274537) at a dilution of 1:1000.
Quick Database Links
Gene Symbol
SQSTM1
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
IP
Immunoprecipitation
SQSTM1 Antibody (orb1274537)
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