SRC Antibody (N-term)

SKU: orb1927590

Description

Research Area

Cancer Biology, Cell Biology, Immunology & Inflammation, Signal Transduction

Images & Validation

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Item 1 of 6

Tested Applications	FC, IF, IHC-P, WB
Dilution Range	WB - 1:2000, IHC-P - 1:100-500, FC - 1:50, IF - 1:50
Reactivity	Human
Predicted Reactivity	Mouse

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Molecular Weight	59835 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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SRC1

Quality Guarantee

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SRC Antibody (N-term) western blot analysis in MCF-7, A431 cell line lysates (35 ug/lane).This demonstrates the SRC antibody detected the SRC protein (arrow).

SRC Antibody (N-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).Alexa Fluor 488-conjugated donkey anti-rabbit lgG secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of SRC Antibody (N-term) with A431 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Confocal immunofluorescent analysis of SRC Antibody (N-term) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

SRC Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SRC Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

SRC Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human small intestine tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SRC Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Quick Database Links

UniProt

P12931

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

Flow Cytometry

Immunofluorescence