Cart summary

You have no items in your shopping cart.

SRI 31215 TFA

SKU: orb1301758

Description

SRI 31215 TFA is a small molecule triplex inhibitor that potently targets matriptase, hepsin, and HGFA, mimicking the function of endogenous HAI-1/2 proteins. It is a valuable research tool for studying HGF/c-Met signaling pathways in cancer metastasis and tissue repair, with applications in both in vitro enzymatic assays and cell-based studies.

Research Area

Signal Transduction

Images & Validation

Key Properties

CAS Number1832686-44-8
MW533.6
Purity99.97% (May vary between batches)
FormulaC27H34F3N5O3
SMILESOC(=O)C(F)(F)F.CC1CN(CC2CCN(Cc3ccccc3)CC2)C(=O)N(C1)c1cccc(c1)C(N)=N
Targetc-Met/HGFR
Solubility10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (3.75 mM);DMSO:25 mg/mL (46.85 mM)

Bioactivity

Target IC50
Hepsin:0.65 μM |HGFA:0.3 μM|Matriptase:0.69 μM
In Vitro
SRI 31215 inhibits fibroblast-induced MET activation, epithelial-mesenchymal transition and migration of cancer cells. SRI 31215 overcomes primary resistance to cetuximab and gefitinib in HGF-producing colon cancer cells and prevents fibroblast-mediated resistance to EGFR inhibitors. Thus, SRI 31215 blocks signaling between cancer cells and fibroblasts and inhibits the tumor-promoting activity of cancer-associated fibroblasts.
Cell Research
DU145 cells were cultured in 6-well tissue culture plates at a density of 1 103 cells per well. After colonies formed (6-8 days), cells were serum-starved overnight and were then treated with recombinant HGF (10 ng/ml) or with conditioned media from 18Co fibroblasts in the presence or absence of SRI 31215 (10 μM) or the MET kinase inhibitor JNJ 38877605 (1 μM) for 24 hours. Cells were washed with PBS and colonies were fixed and stained with 0.5% crystal violet solution in 6% glutaraldehyde.

Storage & Handling

StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

SRI31215, SRI-31215, SRI 31215 TFA, HGFR, HGFA, hepsin, matriptase, c-Met/HGFR, cMet/HGFR, c-Met, cMet
Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Key Properties

No computed properties available.

Documents Download

Datasheet
Product Information
Download

Request a Document

Protocol Information

SRI 31215 TFA (orb1301758)

  • Star
  • Star
  • Star
  • Star
  • Star
  • 0.0
Based on 0 reviews

Participating in our Biorbyt product reviews program enables you to support fellow scientists by sharing your firsthand experience with our products.

Login to Submit a Review

No reviews yet

Step 1: Enter information below

(Recommended: An additional animal making an allowance for loss during the experiment)

Step 2: Enter the in vivo formulation

(This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO +
%+
% Tween 80 +
%

Available Sizes

Select a size below

1 mg
$ 80.00
5 mg
$ 130.00
1 ml x 10 mM (in DMSO)
$ 150.00
10 mg
$ 180.00
25 mg
$ 320.00
50 mg
$ 460.00
100 mg
$ 630.00
200 mg
$ 840.00
DispatchUsually dispatched within 3-5 working days
Bulk Enquiry