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SRI 31215 TFA

SKU: orb1301758

Description

SRI 31215 TFA

Research Area

Signal Transduction

Images & Validation

Key Properties

CAS Number1832686-44-8
MW533.6
Purity99.36%
FormulaC27H34F3N5O3
SMILESOC(=O)C(F)(F)F.CC1CN(CC2CCN(Cc3ccccc3)CC2)C(=O)N(C1)c1cccc(c1)C(N)=N
Targetc-Met/HGFR
Solubility10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (3.75 mM);DMSO:25 mg/mL (46.85 mM)

Bioactivity

Target IC50
Hepsin:0.65 μM |HGFA:0.3 μM|Matriptase:0.69 μM
In Vitro
SRI 31215 inhibits fibroblast-induced MET activation, epithelial-mesenchymal transition and migration of cancer cells. SRI 31215 overcomes Primary resistance to cetuximab and gefitinib in HGF-producing colon cancer cells and prevents fibroblast-mediated resistance to EGFR inhibitors. Thus, SRI 31215 blocks signaling between cancer cells and fibroblasts and inhibit the tumor-promoting activity of cancer-associated fibroblasts.
Cell Research
DU145 cells were cultured in 6-well tissue culture plates at a density of 1 103 cells per well. After colonies formed (6-8 days), cells were serum-starved overnight and were then treated with recombinant HGF (10 ng/mL) or with conditioned media from 18Co fibroblasts in the presence or absence of SRI 31215 (10 μM) o the MET kinase inhibitor JNJ 38877605 (1 μM) for 24 hours. Cells were washed with PBS and colonies were fixed and stained with 0.5% crystal violet solution in 6% glutaraldehyde.

Storage & Handling

StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
DisclaimerFor research use only

Alternative Names

SRI31215, SRI-31215, SRI 31215 TFA, HGFR, HGFA, hepsin, matriptase, c-Met/HGFR, cMet/HGFR, c-Met, cMet
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Key Properties

No computed properties available.

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SRI 31215 TFA (orb1301758)

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% DMSO +
%+
% Tween 80 +
%

Available Sizes

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1 mg
$ 80.00
5 mg
$ 130.00
1 ml x 10 mM (in DMSO)
$ 150.00
10 mg
$ 180.00
25 mg
$ 320.00
50 mg
$ 460.00
100 mg
$ 630.00
200 mg
$ 840.00
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