Stat3 Monoclonal Antibody [Knockout Validated]

SKU: orb1787820

Description

Purified Mouse Monoclonal Antibody (Mab)

Research Area

Cancer Biology, Cardiovascular Research, Immunology & Inflammation, Infectious Disease & Virology, Microbiology, Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

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Item 1 of 2

Tested Applications	WB
Dilution Range	WB: 1:1000, WB: 1:2000
Reactivity	Human
Predicted Reactivity	Mouse, Porcine, Rat

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG2a,κ
Target	This Stat3 antibody is generated from a mouse immunized with Stat3 recombinant protein.
Molecular Weight	88068 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Signal transducer and activator of transcription 3, Acute-phase response factor, STAT3, APRF, knockout

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Stat3 Monoclonal Antibody [Knockout Validated]

Western blot analysis of lysate from Hela cell line, using Stat3 Antibody. Diluted at 1:1000. A goat anti-mouse IgG H&L (HRP) at 1: 3000 dilution was used as the secondary antibody. Lysate at 35μg.

All lanes: Anti-Stat3 Antibody at 1:2000 dilution (upper). Lane 1: Hela. Lane 2: Hela-Stat3-Knockout. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 88 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

This Stat3 antibody is generated from a mouse immunized with Stat3 recombinant protein.

UniProt

P40763

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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