STAT4 Rabbit Polyclonal Antibody

SKU: orb158505

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Description

Research Area

JAK/ STAT Signaling Pathway

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, ICC, WB
Dilution Range	WB=1:500-2000, ICC/IF=1:100-500, ELISA=1:5000-10000
Reactivity	Human
Predicted Reactivity	Bovine, Canine, Human, Mouse, Porcine, Rabbit, Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human STAT4 (111-210/748aa)
Target	STAT4
Molecular Weight	85 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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signal transducers and activators of transduction 4; CIS4; HSPC060; SOCS4; SOCS6; SSI4; STAI4; STAT 4; STAT4; STATI4; Suppressor of cytokine signaling 6; STAT4_HUMAN.

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Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (STAT4) Polyclonal Antibody, Unconjugated (orb158505) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

STAT4

UniProt

Q14765

UniProt Details

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No UniProt data available

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Datasheet

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Protocol Information

Western Blot (IB, immunoblot)

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ICC

Immunocytochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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