STAT5b Recombinant Rabbit Monoclonal Antibody

SKU: orb559145

Description

Research Area

Hypoxia, JAK/ STAT Signaling Pathway

Images & Validation

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Item 1 of 1

Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	12A1
Immunogen	Recombinant human STAT5b protein, around 500-600aa
Target	STAT5B
Molecular Weight	90 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Transcription factor STAT5B; Signal transducer and activator of transcription 5B; STA5B_HUMAN; STAT5.

Quality Guarantee

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STAT5b Recombinant Rabbit Monoclonal Antibody

Blocking buffer: 5% NFDM/TBST, Primary ab, dilution: 1:1000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: HeLa, K562, PC-12, A20, Protein loading quantity: 20 µg, Exposure time: 10 s, Predicted MW: 90 kDa, Observed MW: 90 kDa.

Quick Database Links

Gene Symbol

STAT5B

UniProt

P51692

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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