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STAT6 Antibody
SKU: orb345705
Description
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution Range | ELISA: 1:32,000, WB: 1:1000 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a phosphorylated synthetic peptide corresponding to residues surrounding Tyrosine Y641 of human STAT6 protein. |
| Target | STAT6 |
| Purity | This affinity purified antibody is directed against human STAT6 pY641 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with STAT6 pY641 protein from human at 100% based on homology with the immunizing sequence. Reactivity against homologues from other sources is not known. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1.13 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−rabbit anti-STAT6 pY641 antibody, phosphorylated STAT6 antibody, STAT-6, STAT 6, Signal transducer and activator of transcription 6 antibody, IL-4 Stat, Interleukin-4 Stat
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Immunohistochemistry of Rabbit anti STAT6 pY641 Antibody. Tissue: Human breast carcinoma pH9 (left) with negative control (right).
Western Blot of Rabbit anti-STAT6 pY641 antibody. Lane 1: unstimulated Hela cell lysate (p/n orb348668). Lane 2: IL-4 stimulated Hela cell lysate. Load: 10 µg per lane. Primary antibody: STAT6 pY641 antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP goat anti-rabbit (orb347654) at 1:40000 for 30 min at RT. Block: 5% Blotto (p/n orb348624), for 30 min at RT. Predicted/Observed size: 94 kDa, 90 kDa. Other band(s): unspecific bands.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001171549.1 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
STAT6 Antibody (orb345705)
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