Staurosporine

SKU: orb1300561

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Description

Staurosporine

Research Area

Cell Biology, Stem Cell & Developmental Biology

Images & Validation

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Key Properties

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CAS Number	62996-74-1
MW	466.5
Purity	>98%
Formula	C₂₈H₂₆N₄O₃
SMILES	CN[C@H]1C[C@@H]2O[C@@](C)([C@H]1OC)n1c3ccccc3c3c4CNC(=O)c4c4c5ccccc5n2c4c13
Target	Kinase
Solubility	Soluble in DMSO (up to 50 mg/ml)

Bioactivity

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Target IC50	PKA:15 nM\|PhK:3 nM\|CSK:2000 nM\|PhK (Phosphorylase kinase):3 nM\|PKCε:73 nM\|MLCK:21 nM\|IGF-1R:6150 nM\|ERK1:1500 nM\|PKCα:2 nM\|PKCγ:5 nM\|S6K:5 nM\|CK2:19500 nM\|CK1:163500 nM\|EGFR:100 nM\|PKCη:4 nM\|TPK-IIB/Syk:16 nM\|IR:61 nM\|Ca 2+ /CaM PK-I1:20 nM\|v-Src:6 nM\|cdc2:9 nM\|PKCδ:20 nM\|PKCζ:1086 nM Read more...
In Vivo	METHODS: To assay anti-tumor activity in vivo, Staurosporine (3 mg/kg) and Lapatinib (50 mg/kg) were administered by gavage twice a week for two weeks to Nu/J-Foxn1 Nu/Nu mice harboring human mammary carcinoma tumors JIMT-1. RESULTS: The combination of Staurosporine and Lapatinib inhibited tumor growth in a statistically significant manner. METHODS: To examine the effects on islet β-cell function, Staurosporine (0.4 mg/kg in 0.5% sodium carboxymethyl cellulose) was administered intraperitoneally to iPLA2β-/- C57BL6 mice once daily for two weeks. for two weeks. RESULTS: Staurosporine impairs glucose tolerance and glucose-stimulated insulin secretion in pancreatic islets. Read more...
In Vitro	METHODS: Human cervical cancer cells HeLa were treated with Staurosporine (1-10 nM) for 72 h, and cell viability was measured by MTT. RESULTS: Staurosporine inhibited the proliferation of Hela cells in a dose-dependent manner, with an IC50 of about 10 nM. METHODS: Human pancreatic cancer cells PaTu 8988t and Panc-1 were treated with Staurosporine (1 μM) for 3-24 h, and cell death was detected by Flow Cytometry. RESULTS: For PaTu 8988t cells, incubation with Staurosporine for 3-24 h significantly increased apoptosis and significantly decreased the number of viable cells; necrosis increased after 6-16 h. For Panc-1 cells, Staurosporine treatment significantly increased apoptosis and significantly decreased the number of viable cells after 9-24 h. The RESULTS were summarized as follows. METHODS: Human hepatocellular carcinoma cells HepG2 were treated with Staurosporine (20 nmol/L) for 6-24 h. The expression levels of target proteins were detected by Western Blot. RESULTS: Staurosporine significantly inhibited the phosphorylation of mTOR and increased the expression of LC3-II, an autophagy marker protein, suggesting that Staurosporine activates autophagy effectively by inhibiting mTOR. Read more...
Cell Research	Cells are exposed to Staurosporine for ~32 hours. Cells are fixed in 4% paraformaldehyde and stained with the DNA-binding dye Hoechst 33342. Cells are visualized under epifluorescence illumination, and the percentage of apoptotic cells (cells with condensed and fragmented DNA) is determined. (Only for Reference) Read more...

Storage & Handling

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Storage	-20°C
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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PKCα, PKCγ, PKCη, PKC, PKA, Protein kinase A, Protein kinase C, STS, Staurosporine, Bacterial, Apoptosis, Antibiotic AM 2282, Antibiotic AM2282, Antibiotic AM-2282, Antibiotic, AM 2282, AM2282, AM-2282, c-Fgr, CGP 41251, CGP41251, CGP-41251, Inhibitor, Fungal, inhibit

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Compound Identifiers

PubChem CID

42610657 ↗

Key Properties

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No computed properties available.

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Applications:	ELISA, WB
Reactivity:	Bacteria
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

CAS Number:	27409-30-9
MW:	492.47
Formula:	C₂₄H₂₈O₁₁

CAS Number:	23720-80-1
MW:	250.33
Formula:	C₁₅H₂₂O₃

CAS Number:	220038-19-7
MW:	470.56
Formula:	C₂₈H₃₀N₄O₃

CAS Number:	120685-17-8
MW:	566.65
Formula:	C₃₃H₃₄N₄O₅

Staurosporine

Description

Research Area

Images & Validation

Key Properties

Bioactivity

Storage & Handling

Alternative Names

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