SUMO1 Antibody (N-term)

SKU: orb1937652

Description

Research Area

Cardiovascular Research, Cell Biology, Infectious Disease & Virology, Metabolism Research

Images & Validation

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Item 1 of 4

Tested Applications	FC, IF, IHC-P, WB
Dilution range	WB - 1:1000, IHC-P - 1:100-500, FC - 1:10-50, IF - 1:10-50
Reactivity	Human
Predicted Reactivity	Bovine, Mouse, Porcine, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Molecular Weight	11557 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Disclaimer	For research use only

Alternative Names

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SMT3C, SMT3H3, UBL1

Quality Guarantee

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Confocal immunofluorescent analysis of SUMO1 Antibody (N-term) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

SUMO1 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

The anti-SUMO1 polyclonal antibody is used in Western blot to detect SUMO1 in HL-60 cell lysate.

Quick Database Links

UniProt

P63165

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

Flow Cytometry

Immunofluorescence