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SUN2 Recombinant Rabbit Monoclonal Antibody
SKU: orb1499408
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Description
Images & Validation
−Item 1 of 6
| Tested Applications | ICC, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution range | WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, ICC/IF=1:50-200, IF=1:50-200 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Immunogen | Recombinant protein within human SUN2 aa 500-700. |
| Target | SUN2 |
| Molecular Weight | 80 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−FRIGG; KIAA0668; nuclear envelope protein; Protein unc-84 homolog B; Rab5 interacting protein; RAB5IP; Sad1 and UNC84 domain containing 2; Sad1 unc-84 domain protein 2; Sad1 unc84 domain protein; Sad1/unc-84 protein-like 2; Sad1/unc84 protein-like; SUN domain-containing protein 2; UNC 84B; unc84 homolog B(C. elegans); unc84 homolog B; unc84; C. elegans; homolog of; B; UNC84B
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ICC staining SUN2 (green) in SK-Br-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-SUN2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-SUN2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-SUN2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-SUN2 antibody. Counter stained with hematoxylin.
Western blot analysis of SUN2 on A431 cell lysates with Rabbit anti-SUN2 antibody (orb1499408) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 80 kDa, Observed band size: 75 kDa, Exposure time: 2 minutes, 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (orb1499408) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300000 dilution was used for 1 hour at room temperature.
Quick Database Links
Gene Symbol
SUN2
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
ICC
Immunocytochemistry
SUN2 Recombinant Rabbit Monoclonal Antibody (orb1499408)
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